DFI (DNA Fragmentation Index)

🔍 What is DFI?

DFI (DNA Fragmentation Index) measures the percentage of sperm with damaged or fragmented DNA strands. While standard semen analysis focuses on count, motility, and shape, the DFI test goes deeper — evaluating the genetic integrity of sperm. High DFI levels can lead to poor embryo quality, implantation failure, or early miscarriages.

⚠️ Why is DFI Testing Important?

• Identifies hidden causes of infertility in men with normal semen reports.
• Helps explain recurrent IVF or ICSI failure despite good embryo quality.
• Detects risk of miscarriages due to damaged sperm DNA.
• Assists in choosing the right treatment — antioxidants, lifestyle changes, or advanced sperm selection techniques.

🧔 Causes of High DNA Fragmentation

• Oxidative stress or high levels of free radicals
• Varicocele (enlarged veins in scrotum)
• Smoking, alcohol, and unhealthy diet
• Exposure to heat, radiation, or environmental toxins
• Age-related sperm damage
• Infections or inflammation in the reproductive tract
• Prolonged abstinence or poor lifestyle habits

🧫 How is DFI Test Performed?

A semen sample is collected and analyzed using advanced laboratory techniques like:

• SCSA (Sperm Chromatin Structure Assay)
• TUNEL Assay (Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling)
• Comet Assay
• SCD (Sperm Chromatin Dispersion) Test

These tests assess the degree of DNA breakage and calculate the DFI percentage. A DFI below 15% is considered normal, 15–25% is borderline, and above 25% indicates high DNA damage.

💊 Treatment & Management for High DFI

• Antioxidant therapy: Supplements like Vitamin C, E, CoQ10, and Zinc reduce oxidative stress.
• Lifestyle modification: Quitting smoking, reducing alcohol, maintaining a healthy diet, and regular exercise.
• Treating underlying conditions: Surgical correction of varicocele or infection management.
• Advanced ART techniques: Use of PICSI (Physiological ICSI) or Micro TESE to select sperm with intact DNA.

🏥 DFI Testing at Phular IVF Hospital, Muzaffarpur

At Phular IVF Hospital, we use the latest DFI testing methods with advanced andrology laboratory facilities. Our fertility specialists provide detailed counseling and customized treatment plans based on the results. With early diagnosis and proper management, couples can significantly improve their chances of natural conception or IVF success.